fusion protein (Bio-Rad)

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Bio-Rad fusion protein
Fusion Protein, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 860 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/affi+gel+15/Affi-Gel+15+Gel/pmc13147537-108-20-29
Average 95 stars, based on 860 article reviews

fusion protein - by Bioz Stars, 2026-09

95/100 stars

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Article Title: Modeling linear and cyclic PKS intermediates through atom replacement
Article Snippet: It was necessary to “apofy” the actACP mixture by tumbling 10 mL of 300 μM ACP in 150 mM NaCl, 50 mM Tris pH 8.0, 15 mM MgCl2, 1 mM MnCl2, 3 mM tris(2-carboxyethyl)phosphine (TCEP), and 250 μL of Affi-gel 15 (Bio-Rad) containing 4 mg/mL covalently-attached PfAcpH discussed in section C.2 at 37 °C for 12 h [Kosa, N. M.; Haushalter, R. W.; Smith, A. R.; Burkart, M. D. Nat.

Article Title: Plasmodium vivax Merozoite Surface Protein-3 (PvMSP3): Expression of an 11 Member Multigene Family in Blood-Stage Parasites
Article Snippet: Thus, the antisera were adsorbed with highly reactive PvMSP3 recombinants coupled to Affi-Gel 15 (Bio-Rad).

Article Title: Broad-Spectrum Inhibitors for Conserved Unique Phosphoethanolamine Methyltransferases in Parasitic Nematodes Possess Anthelmintic Efficacy.
Article Snippet: Briefly, 8 mL of slurry of Affi-Gel 15 (Bio-Rad) was washed twice with 50 mL of distilled water, followed by two washes in 50 mM NaHCO3 buffer.

Article Title: Broad-Spectrum Inhibitors for Conserved Unique Phosphoethanolamine Methyltransferases in Parasitic Nematodes Possess Anthelmintic Efficacy
Article Snippet: Briefly, 8 mL of slurry of Affi-Gel 15 (Bio-Rad) was washed twice with 50 mL of distilled water, followed by two washes in 50 mM NaHCO 3 buffer.

Affinity Purification:

Article Title: The DNA polymerase activity of Pol ε holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts
Article Snippet: The 5'-terminal sequence was obtained by 5'-Full RACE Core Set (TAKARA, Japan) using Xenopus ovary mRNA as templates. .. Rabbit anti- Xenopus Pol ε p60 antibodies were affinity-purified as published [ ] and used for making antigen-immobilized Affi-Gel 15 (Bio-Rad). .. The purified p60 antibodies or whole rabbit IgG (Pierce) as a control was crosslinked to Affi-Prep Protein A matrix (Bio-Rad) (1 mg of IgG per ml of matrix) and used for immunoprecipitation and immunodepletion experiments.

Purification:

Article Title: HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2
Article Snippet: Antibody was provided by Covance in the form of antisera. .. The HURP antibody was then purified by first conjugating 10 mg of HURP(462-576) antigen to 0.5 mL of Affi-gel 15 (Bio-Rad: 1536051) resin (brought up to 10 mL with PBS buffer), rotating overnight at 4 °C. ..

Buffer Exchange:

Article Title: Cell-based method for determining an activity of botulinum toxin
Article Snippet: Preparation of SNAP25-Affinity Column Recombinant SNAP25197 was concentrated to 10 mg/ml using AMICON Ultra-15 by repeated centrifugation at 1,000×g for 10 min at 4° C., while measuring the protein concentration using the Nano spectrophotometer (Drawell Scientific Instrument Co., Ltd, Shanghai). .. This buffer exchange was repeated 6 times, and SANP25197 concentrate was added to 1 ml slurry of Affi-Gel 15 (Bio-Rad) in deionized H2O. .. This buffer exchange was repeated 6 times, and SANP25197 concentrate was added to 1 ml slurry of Affi-Gel 15 (Bio-Rad) in deionized H2O.

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